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KMID : 0545119980080020146
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 2 p.146 ~ p.151
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Cloning and Sequence Analysis of the Aminoglycoside Resistance Gene from a Nebramycin Complex Producer, Streptoalloteichus hindustanus
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Hyun, Chang Gu
Kim, Jong Woo/Han, Jae Jin/Choi, Young Nae/Suh, Joo Won
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Abstract
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The aminoglycoside multiple-resistance determinant from Streptoalloteichus hindustanus was cloned into Streptomyces lividans and named nbrB. The 1.2-kb ApaI-BclI fragment encompassing nbrB was located within a 2.6-kb ApaI fragment by successive subcloning experiments. The complete DNA nucleotide sequence of 1.2-kb containing nbrB was determined. The sequence contains an open reading frame that putatively encodes a polypeptide of 281 amino acids with a predicted molecular weight of 30,992. The deduced amino acid sequence of nbrB shows identities of 85.1% to kgmB of S. tenebrarius, 59.6% to sgm of Micromonospora zionensis, and 57.7% to grm of M. rosea. The similarity of nbrB to kgmB suggests that nbrB encodes a 16S rRNA methylase similar to that encoded by kgmB and that both genes might be derived from a common ancestral gene.
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